More: Apple Podcasts | ESPN App | RSS | Embed Embed Code This week, Hot Takedown brings you an episode made up entirely of Rabbit Holes.To celebrate the English Premier League kicking off this week, we discover Geoff’s solution to what are, in his opinion, terrible fantasy soccer leagues.Sara examines the unparalleled density of national champions in the WNBA, and Neil searches for long-lost NASCAR road course ringers.To wrap, we recap the record-breaking events of the 2019 X Games, debate whether esports are indeed sports and look back at extreme sports of the past.A few videos we can’t stop watching:Past street luge racesGold medal skysurfing jumpsCompetitive bungee jumpingBarefoot water ski jumping FiveThirtyEight
(Phys.org)—A team of researchers with Massachusetts General Hospital, Harvard Medical School and the Broad Institute has developed a procedure to decode histone modifications that point out gene regulation. In their paper published in the journal Science, the team describes the approach they developed to map combinations of modifications made to individual nucleosomes. Altered primary chromatin structures and their implications in cancer development Determining whether a gene should be active or not has been linked to covalent modification of histone proteins that form the framework upon which DNA strands are wound. Scientists would like to better understand how the coding works but have not been able to find a way to uncover the combinations at play, or to quantify or map them. In this new effort, the researchers report that they have developed a technique that allows for doing just that. Their strategy involved starting with pluripotent stem cells as well as lineage-committed cells, singling out nucleosomes (the packages that contain histone proteins and DNA strands) and dabbing the ends of DNA strands with fluorescent material, meanwhile the DNA was incubated with histones that had been flagged with fluorescent antibodies. Next to pinpoint individual nucleosomes, the team used internal reflection microscopy which allowed for creating millions of images which set the stage for decoding modification states. In so doing, the team was able to actually quantify different changes on single nucleosomes, which led to demonstrating that genetic and chemical changes preferentially impacted nucleosomes with certain modification states. The last step involved applying the same approach to single-molecule DNA sequencing to figure out the exact location of the modified nucleosomes in a given genome.In using their technique, the team reports that they found evidence that suggested that cells that had been differentiated showed different patterns of “bivalent” markings than did embryonic cells. They suggest that the technique holds promise of assisting in answering some of the fundamental questions in both chromatin biology and epigenetic regulation by providing new insights into genetic processes. They also note that their results represent early results in their efforts to better understand histone signaling regulation and expect that eventually they will have a more complete understanding of the rules that underlie the fate of cells in general. Explore further © 2016 Phys.org More information: E. Shema et al. Single-molecule decoding of combinatorially modified nucleosomes, Science (2016). DOI: 10.1126/science.aad7701AbstractDifferent combinations of histone modifications have been proposed to signal distinct gene regulatory functions, but this area is poorly addressed by existing technologies. We applied high-throughput single-molecule imaging to decode combinatorial modifications on millions of individual nucleosomes from pluripotent stem cells and lineage-committed cells. We identified definitively bivalent nucleosomes with concomitant repressive and activating marks, as well as other combinatorial modification states whose prevalence varies with developmental potency. We showed that genetic and chemical perturbations of chromatin enzymes preferentially affect nucleosomes harboring specific modification states. Last, we combined this proteomic platform with single-molecule DNA sequencing technology to simultaneously determine the modification states and genomic positions of individual nucleosomes. This single-molecule technology has the potential to address fundamental questions in chromatin biology and epigenetic regulation. Journal information: Science Citation: Procedure developed to decode histone changes that point out gene regulation (2016, May 10) retrieved 18 August 2019 from https://phys.org/news/2016-05-procedure-decode-histone-gene.html Nucleosome core particle, crystal structure (PDB ID: 1EQZ). Credit: Darekk2, Protein Data Bank (PDB), via Wikipedia This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
MONTREAL — Air Canada Vacations is making good on its promise to overhaul its digital platforms with an upgrade of its ISO platform, to facilitate dynamic packaging as well as ancillary sales.Air Canada Vacations says it’s upgrading its existing ISO Platform, Ocean, to the new booking engine, Pacific.The switch will take place over the next 12 months with adaptive integration options for future systems, says ACV, as well as a more user-friendly interface. In tandem, ISO Travel Solutions and Air Canada Vacations will migrate the passenger service system to Amadeus’ Altéa.Air Canada Vacations’ partnership with ISO Travel Solutions dates back to 2005.“We’re pleased to continue our longstanding partnership with ISO Travel Solutions,” says Nino Montagnese, ACV’s Managing Director. “The Pacific platform offers us the ultimate flexibility to continue to fulfill the needs of our customers. The streamlined and intuitive booking engine helps us to deliver our carefully curated vacations to our customers for all styles of travel.”More news: A new low for no-frills flying: easyJet assigns backless seat to passengerIn addition to what ACV says is a scalable and easy-to-use interface for call centres, Pacific also offers features to enable dynamic packaging while managing various travel products with its versatile inventory management system. ACV says the platform also provides distribution strategies with multiple sales channels and allows for adaptive pricing of ancillary sales.“We are happy to deliver our powerful and proven travel platform, Pacific, to Air Canada Vacations,” says Holger Voss, ISO’s business development director. “The upgrade to Pacific will provide a contemporary, web-based reservation system. Pacific’s advanced features enable Air Canada Vacations to manage, sell and service not only pre- and dynamic-packaged travels, but also FIT, custom tours, cruises groups and more using internal and external connected inventories.”Last month ACV announced a new initiative aimed at the complete transformation of its digital platforms. ACV prioritizes dynamic packaging, ancillary sales with tech upgrade Share