Research demonstrates Parsortix workflow for culturing circulating tumour cells for in-vitro drug testing

first_img Menopause to become the next game-changer in global femtech solutions industry by 2025 WHO tri-regional policy dialogue seeks solutions to challenges facing international mobility of health professionals By EH News Bureau on April 25, 2018 The research was undertaken by the Robert H Lurie Comprehensive Cancer Centre and the Feinberg School of Medicine, Northwestern University, Chicago (Northwestern)Unique capabilities of ANGLE’s Parsortix system address a key aim of precision medicine to test drugs outside the patient to determine which drugs will benefit the patient Work highlights another major opportunity for the use of ANGLE’s Parsortix system in breast cancer post FDA clearanceANGLE plc, a liquid biopsy company, announced that results presented by one of the leading US cancer centres at the American Association for Cancer Research conference (AACR) 2018, in Chicago have demonstrated a workflow for culturing circulating tumor cells (CTCs) using ANGLE’s ParsortixTM system. The research undertaken by the Robert H Lurie Comprehensive Cancer Centre and the Feinberg School of Medicine, Northwestern University, Chicago (Northwestern) has developed an optimised workflow for the recovery and culturing of CTCs from a simple blood test to produce an effective ex-vivo culture (cells growing outside the patient) of the individual patient’s cancer cells.The team of investigators led by Dr Massimo Cristofanilli focussed on demonstrating the capability to interrogate and test cancer cells collected from patients with advanced breast cancer. This was only possible because the epitope-independent (does not use antibodies) patented microfluidic process used by the Parsortix system for CTC enrichment harvests undamaged living CTCs, which can then be cultured. Reportedly, this is the first time that an optimised workflow has been presented to culture CTCs ex-vivo. This has been a goal of many research groups for some time but has not been achievable because the methods of CTC isolation used yield low numbers of only partially purified CTCs that are fixed before isolation (hence killed), damaged during the cell purification process, or irreversibly immobilised on an adherent matrix.The Parsortix system neatly avoids all these problems providing a simple reproducible way to harvest CTCs that can be cultured. This is another example of the use of CTCs harvested using the Parsortix system, which cannot be addressed using ctDNA. Previous culturing of CTCs harvested from leukapheresis (transfusion of blood out and back into the body taking up to an hour) product using Parsortix has been reported but this is the first time it has been achieved from a simple blood test. Northwestern tested their ex-vivo culture workflow on 16 metastatic breast cancer patients. A single 7.5ml EDTA blood tube was drawn from a simple blood test for each patient. In every patient (100 per cent), the Parsortix system yielded viable CTCs that could be developed into an ex-vivo culture. The number of CTCs harvested using the Parsortix system was significantly higher than has been reported with other systems and ranged from 300 to 17,250 CTCs per patient. The CTCs were then successfully cultured over a three week period at which time the DNA was isolated and purified. The CTCs in culture retained their morphology and expanded 3.5 – 5.5 fold in week one, and then 9.5 – 22.5 fold in week two yielding as many as 100,000 cancer cells within 14 days thus providing sufficient DNA for a wide range of multiple analyses to be undertaken.Northwestern’s poster presentation to AACR 2018 is entitled “A novel ex vivo culture workflow to enrich and expand circulating tumor cells (CTCs) from patients with Stage III/IV Breast Cancer (BCa) (LB-370)”. Consequently a non-invasive method to provide a series of ex-vivo cultures of the patient’s cancer cells for organoids development and in-vitro drug testing enables the changing patterns of drug susceptibility in individual patients to be monitored as their tumours acquire new mutations. This would enable the patient to receive the right drug at the right time, improving outcomes for patients and avoiding wasted resources on drugs, which are ineffective. Professor Massimo Cristofanilli, MD Associate Director, Translational Research – Robert H Lurie Comprehensive Cancer Centre, Northwestern University, commented, “The ability to recover, culture and interrogate cancer cells expands our possibility to advance precision medicine in breast cancer patients. We believe that the optimisation of the workflow utilising the Parsortix system to produce ex-vivo cultures of CTCs is already opening up a new frontier in the management of breast cancer, by allowing the testing of treatments in a ‘predictive in vitro system’ truly representative of the patient’s disease and improving our ability to select agents and predict efficacy. Widely adopted, this approach has the potential to transform the way we treat cancer patients.”Andrew Newland, ANGLE Founder and Chief Executive, commented, “This is the first time that CTCs have been successfully cultured direct from a Parsortix processed whole blood sample. This work is highly encouraging for ANGLE. Firstly every metastatic breast sample analysed yielded a large number of CTCs, which is a positive sign for our FDA study. Secondly, Northwestern has achieved something that many other Centres before them have failed to do, which is to produce an optimised workflow for reproducibly growing the CTCs. This approach is potentially applicable for every metastatic breast cancer patient and provides another clear high value application for ANGLE’s Parsortix system.” Indraprastha Apollo Hospitals releases first “Comprehensive Textbook of COVID-19” Phoenix Business Consulting invests in telehealth platform Healpha Related Posts Sharecenter_img MaxiVision Eye Hospitals launches “Mucormycosis Early Detection Centre” The missing informal workers in India’s vaccine story Read Article Heartfulness group of organisations launches ‘Healthcare by Heartfulness’ COVID care app Research demonstrates Parsortix workflow for culturing circulating tumour cells for in-vitro drug testing News last_img read more

Project xCloud expands with 50 new titles, coming to Japan, India and more next year

first_imgProject xCloud expands with 50 new titles, coming to Japan, India and more next yearXbox’s streaming service will be incorporated into Xbox Game Pass; Bluetooth controller support coming next yearJames BatchelorEditor-in-ChiefThursday 14th November 2019Share this article Recommend Tweet ShareCompanies in this articleMicrosoftXboxMicrosoft gave an update on the progress of its Project xCloud streaming service at X019 in London today, with 50 new titles coming to the current preview service.So far, preview players in Korea, the UK, and US have only had access to a handful of first-party games, including Gears 5, Sea of Thieves and Killer Instinct. As the preview enters its second month, Microsoft is not only adding more of its own games — including Crackdown 3, Forza Horizon 4 and Ori and the Blind Forest — but also titles from 25 third-party publishers and developers.Highlights include Madden NFL 20, WWE 2K20, Hitman, Just Cause 4, and Devil May Cry 5. You can find a full list of titles below.Additional invites to the preview are now being sent out to Xbox gamers who registered their interest, with the promise of more invites in the future.Microsoft also announced it will be bringing Project xCloud Preview to more markets in 2020, including Canada, India, Japan, and Western Europe.Next year will also see the service brought to Windows 10 PCs, with Microsoft “collaborating with a broad set of partners to make game streaming available on other devices as well.”Related JobsSenior Game Designer – UE4 – AAA United Kingdom Amiqus GamesProgrammer – REMOTE – work with industry veterans! North West Amiqus GamesJunior Video Editor – GLOBAL publisher United Kingdom Amiqus GamesDiscover more jobs in games The company has emphasised that it wants xCloud to offer more than just playing Xbox games on phones or tablets, so is also expanding the number of input devices users can play with. Starting from next year, Project xCloud will support Bluetooth game pads from Razer and even the PlayStation 4’s DualShock 4 controller.There are also plans to enable Xbox owners to stream any games they already own, and this option will be enabled with future purchases. Xbox Game Pass subscribers will also have the ability to stream titles from that collection.Here is the full list of additional preview titles:Absolver (Devolver Digital)Ace Combat 7: Skies Unknown (Bandai Namco) Ark: Survival Evovled (Studio Wildcard)Battle Chasers: Nightwar (THQ Nordic)Black Desert Online (Pearl Abyss)Bloodstained: Ritual of the Night (505 Games)Borderlands: The Handsome Collection (2K Games)Brothers: A Tale of Two Sons (505 Games)Conan Exiles (Funcom)Crackdown (Xbox Game Studios)Darksiders 3 (THQ Nordic)Dead Island: Definitive Edition (Koch Media)Devil May Cry 5 (Capcom)F1 2019 (Codemasters)For The King (Curve Digital) Forza Horizon 4 (Xbox Game Studios)Gears of War: Ultimate Edition (Xbox Game Studios)Halo Wars 2 (Xbox Game Studios)Hellblade: Senua’s Sacrifice (Xbox Game Studios)Hello Neighbour (TinyBuild)Hitman (IO Interactive)Just Cause 4 (Square Enix)Madden NFL 20 (Electronic Arts)Mark of the Ninja: Remastered (Klei Entertainment)Mutant Year Zero (Funcom)Ori and the Blind Forest: Definitive Edition (Xbox Game Studios)Overcooked (Team17)Puyo Puyo Champions (Sega)RAD (Bandai Namco)Recore: Definitive Edition (Xbox Game Studios)Shadow of the Tomb Raider: Definitive Edition (Square Enix)Sniper Elite 4 (Rebellion)SoulCalibur VI (Bandai Namco)State of Decay 2 (Xbox Game Studios)Subnautica (Unknown Worlds)Tales of Vesperia: Definitive Edition (Bandai Namco)Tekken 7 (Bandai Namco)Tera (Krafton)The Bard’s Tale IV: Director’s Cut (Xbox Game Studios)theHunter: Call of the Wild (Avalanche Studios)Vampyr (Focus Home Interactive)Vermintide 2 (Fatshark Games)World of Final Fantasy Maxima (Square Enix)World of Tanks: Mercenaries (Wargaming)World of Warships: Legends (Wargaming)World War Z (Mad Dog Games)WRC 7 (Bigben Interactive)WWE 2K20 (2K Games)Yoku’s Island Express (Team17)Celebrating employer excellence in the video games industry8th July 2021Submit your company Sign up for The Publishing & Retail newsletter and get the best of in your inbox. Enter your email addressMore storiesEA leans on Apex Legends and live services in fourth quarterQ4 and full year revenues close to flat and profits take a tumble, but publisher’s bookings still up double-digitsBy Brendan Sinclair An hour agoEA Play Live set for July 22Formerly E3-adjacent event moves to take place a month and half after the ESA’s showBy Jeffrey Rousseau 3 hours agoLatest comments Sign in to contributeEmail addressPasswordSign in Need an account? Register now.last_img read more

Suicide Six to open five days a week

first_imgSuicide Six Ski Area,Woodstock Inn & Resort,The Woodstock Inn & Resort Announces Modified Operations for 2020-2021 Season at Suicide Six Ski Area and the Nordic CenterVermont Business Magazine For its 85th season, the Woodstock Inn & Resort’s Suicide Six Ski Area(link is external) will modify operations to prioritize the safety of guests and employees while also ensuring a quality snow experience that are hallmarks of the mountain.“The changes for the 2020-2021 ski season are a necessary shift to protect the health and safety of our patrons and employees,” said Tim Reiter, general manager of Suicide Six Ski Area. “We are excited to provide our valued guests a stellar snow experience while also safeguarding their wellbeing. As winter progresses, we will continue to follow the guidance of public health experts and state government officials.”The operating schedule will be modified to five days per week, opening the lodge and lifts from Wednesday through Sunday. Every guest must complete a ski attestation form(link is external) upon arrival to certify compliance with all state guidelines. Face coverings are required for guests and employees at all times in public spaces such as outdoor seating areas, the parking lot, in the lodge, loading and riding the lifts, and during all ski and snowboard lessons. Masks can be taken down while on the trails or eating.The lodge will have usage and interior capacity limits with access only permitted to the ski shop, equipment rentals, and restrooms. Temperature screening will be mandatory prior to entrance into the base lodge. Movement patterns will be employed and clearly defined to promote social distancing. Storage of personal belongings will not be permitted inside the lodge and patrons’ vehicles will become their lockers.This season all food and beverage services will be offered outside. To ensure a safe, distanced après ski experience Perley’s Pourhouse will serve chef-prepared grab-and-go-meals and to-go bar service. The ski area’s expanded outdoor deck features additional seating, fire pits, and wind cover areas for guests to rest and warm up.Pass prices have been kept at last year’s reduced rate. Ski pass buyers are guaranteed that if the season becomes limited to 30 operating days or less, passes can roll forward to 2021-2022. Daily lift tickets may be limited at peak periods and guests are encouraged to purchase tickets online in advance to minimize in-person transactions. The ski area’s new Heroes Pass will honor active-duty military, veterans, and their spouses, as well as all healthcare and healthcare support workers.Vermont’s leading family ski resort is home to 100 acres of skiing and snowboarding on 24 trails suitable for all skill levels. Compared to larger Northeast ski areas, Suicide Six offers shorter than average lift times and less crowds. Full details of the modified operations at Suicide Six Ski area can be found here: is external).The Woodstock Inn & Resort Nordic Center(link is external) provides 45-kilometers of groomed trails surrounding Mt. Peg. Guests can explore the trail network on Nordic skis, fatbikes, or snowshoes. The Nordic Center lodge will have an expanded footprint, allowing for more space in the retail section and additional guest comfort areas. Face coverings will be required for employees and guests in all areas of the Nordic Center, with the exception of while participating in activities on the trails. Trail tickets may be limited at peak periods, and guests are encouraged to purchase online in order to limit in-person transactions.All out-of-state visitors in Vermont must adhere to the latest quarantine requirements(link is external) and are required to are required to fill out a Vermont Certificate of Compliance(link is external).About Suicide Six Ski AreaRecognized as one of the oldest ski areas in the country, Suicide Six has a rich history. America’s first rope tow, introduced on nearby Gilbert’s Farm in 1934, evolved into the opening of Suicide Six in 1936. In 1961 Laurance S. Rockefeller purchased Suicide Six from its founder, Wallace “Bunny” Bertram, as part of his vision for the community of Woodstock. Suicide Six is one of Vermont’s leading family-friendly ski resorts featuring more than 100 acres of skiing on 24 trails with terrain suitable for all abilities.About the Woodstock Inn & ResortLocated in idyllic Woodstock, Vermont, the Woodstock Inn & Resort defines country sophistication in one of New England’s most charming and popular year-round vacation destinations.  The 142-room, AAA Four Diamond Resort and a member of the Preferred Hotels & Resorts LVX Collection, offers award-winning dining in two restaurants, ranked among the finest in New England.  The Woodstock Inn & Resort is owned and operated by The Woodstock Foundation, Inc.  Proceeds from Resort operations support The Woodstock Foundation and Billings Farm & Museum education and conservation programs. For more information about the resort visit: is external).About Preferred Hotels & ResortsThe Woodstock Inn & Resort is a member of the Preferred Hotels & Resorts Lifestyle Collection, which represents premier global properties that offer engaging stays and memorable moments. Authentic, intelligent, and approachable, this diverse collection presents hotels and resorts featuring responsive service and local dining that articulate culture and style. For more information, visit is external).Source: WOODSTOCK, VT (December 10, 2020) Woodstock Inn & Resortlast_img read more

Browns Name Gregg Williams Interim Head Coach

first_imgBEREA –  The Cleveland Browns have reportedly named Defensive Coordinator Gregg Williams Interim Head Coach.This comes after the team fired Hue Jackson earlier today.  Williams will be taking over amidst a three game losing streak by the Browns.It was reported earlier that Senior offensive assistant Al Saunders was taking over for Jackson, but now it’s Williams’ show to run. Related TopicsGregg Williams Patrick Kennedylast_img

Numbers game: Barnsley (H)

first_imgNiclas Eliasson starred in the late show at Ashton Gate, with his 87th-minute goal enough to put City level on points with the top-six chasers.His strike secured the 1-0 win over Barnsley on Saturday, steering the ball home in front of the South Stand after a strong run and cross from Jay Dasilva.It was one of those games which, as it progressed, always felt it would be settled by the narrowest of margins with both sides fighting to keep pressure at differing ends of the table.The Robins and the Tykes even hit the crossbar and missed a host of chances, before Eliasson pounced to the lift City to eighth in the Sky Bet Championship.TEAM STATSShots: 10Goals: 1Total passes: 299Pass success rate: 62%Aerials won: 31Tackles: 16Interceptions: 10Clearances: 23Possession: 42%last_img